Evaluation Microrna-330 Gene Expression In Human Papillomavirus Type 16-Associated Prostate Cancer Patients Using RT-QPCR

Abstract

Background: Human Papillomavirus (HPV) type 16 is one of the most common sexually transmitted viruses, suggesting a potential long-standing link to prostate cancer. The viral genome has been seen as most demonstrated effect to prostate carcinogenesis and levels of miRNA-330 expression, ignoring the capability of miRNA-330 in tumor-suppression role by regulating cell death and proliferation pathways, Human Papillomavirus type 16 kept the tumor activity. This inapparent functional reversal or equal could lead to different results depending on the potency of the virus to produce the oncogenic proteins, Because of the unknown influence of these factors, given the limited number of studies linking these two factors. Objective: the aim of this study is to find the relationship between the Human Papillomavirus type 16 infection and develop of the prostate cancer in the sphere of influence of the microRNA-330. Methods: the study has conducted in a cohort of prostate cancer patients after the samples were isolated from the Hospital of the (Middle Euphrates Cancer Center), ranging from 47 to 98 years old (n = 100), and divided into two group (PCa with HPV16 and PCa without HPV16). quantified the levels of viral genome and miRNA-330 were done by RT-qPCR. Results: The results showed a positive correlation, with a P-value < 0.05, indicating a clear correlational effect of latent human Papillomavirus type 16 infection. This correlation was further supported by the high gene expression rate of miRNA-330 about 5.27 ± (1.3) in PCa patients with HPV16, while 1 ± (0.22) in PCa patients without HPV16. Conclusions: This study provides novel insights into the nature and progression of prostate tumors and the role of miRNA-330 as a biomarker. The study took place in Najaf, Iraq.