HPTLC Analysis And Phytochemical Profiling of Extract of Cryptocoryne Spiralis

Abstract

Background and Objectives: Cryptocoryne spiralis (Araceae) is a famous aquatic plant used to treat diarrhoea and other diseases. This work evaluated Cryptocoryne spiralis leaf and rhizome extract phytochemical elements and created an HPTLC fingerprint profile. The goal was to identify important bioactive chemicals and create chromatographic fingerprints for plant material quality monitoring and standardisation. The plant has carbohydrates, proteins, phenolics, flavonoids, saponins, glycosides, alkaloids, sterols, fixed oils, and lipids.

Material and Methods: Methanol and hexane solvents were used to gather, shade-dry, grind, and extract fresh Cryptocoryne spiralis leaves and rhizomes. In order to identify carbohydrates, proteins, phenolics, flavonoids, alkaloids, glycosides, saponins, sterols, and fixed oils, preliminary phytochemical screening was done using standard qualitative techniques. Spectrophotometric analysis was used to quantify the total phenolic content, total flavonoid content, and starch content. Using optimised mobile phases, HPTLC analysis was performed on silica gel 60 F254 plates. To acquire distinctive fingerprint profiles, chromatograms were examined under UV light at 254 nm and 366 nm.

Results: Both leaf and rhizome extracts were found to include carbohydrates, proteins, phenolics, flavonoids, saponins, glycosides, alkaloids, sterols, fixed oils, and lipids, according to phytochemical analysis. It was discovered that the total flavonoid content was 6.58 ± 0.27 mg RE/g extract and the total phenolic content was 18.61 ± 0.42 mg GAE/g extract. Rhizomes had a greater starch content (12.84 ± 0.76%) than leaves (7.46 ± 0.54%). Different chromatographic patterns with several resolved peaks were identified by HPTLC fingerprinting. There were seven main peaks in the rhizome methanolic extract and eleven in the hexane extract. The existence of various phytochemical elements was indicated by the 12 peaks seen in the leaf methanolic extract and the 11 peaks seen in the matching hexane extract. The produced fingerprints showed repeatable Rf values and peak regions appropriate for plant material authenticity and quality evaluation.

Conclusion: According to the study, Cryptocoryne spiralis is a rich source of bioactive phytochemicals, including flavonoids and phenolics. For the purpose of characterising and standardising the plant extracts, HPTLC fingerprint profiling offered a dependable and repeatable technique. Future pharmacological studies of Cryptocoryne spiralis may use the established chromatographic profile as a reference for quality assurance and verification.