Antibacterial and Antioxidant Efficacy of Lignocaine-Enhanced Antibiotic-Infused Collagen Membranes: A Comparative in Vitro Evaluation For Oral Wound Healing

Abstract

Objective:
To comparatively evaluate the antimicrobial and antioxidant properties of collagen membranes infused with antibiotic alone versus antibiotic combined with lignocaine for potential use in enhanced wound healing.

Methods:
Collagen membranes were infused with antibiotic (Group A) or antibiotic with lignocaine (Group B) and evaluated in vitro. Antimicrobial activity was assessed using agar diffusion against Streptococcus mutans, Lactobacillus spp., Staphylococcus aureus, Candida albicans, and Enterococcus faecalis. Antioxidant activity was determined using DPPH, hydrogen peroxide, FRAP, ABTS, and nitric oxide scavenging assays.

Results:
Both groups demonstrated dose-dependent antimicrobial activity against S. mutans, Lactobacillus spp., and S. aureus, with no significant activity against C. albicans or E. faecalis. Antioxidant assays revealed strong radical-scavenging activity in both groups, comparable to standard controls, with slightly higher activity observed in Group B.

Conclusion:
Antibiotic-infused collagen membranes, with or without lignocaine, exhibit targeted antimicrobial action along with significant antioxidant properties. The addition of lignocaine enhances overall bioactivity, supporting its potential role in multifunctional biomaterials for oral and periodontal regeneration.