In Vitro Evaluation of The Biocompatibility and Mass-Loss Behaviour of A PVA–CMC-Based Wound-Dressing Film Incorporating Colloidal Silver Gel

Abstract

Background: Biological compatibility and mass-loss behaviour are important preliminary properties of materials being investigated for wound-dressing applications. This study describes an in-vitro assessment of a polyvinyl alcohol (PVA)–carboxymethyl cellulose (CMC) film incorporating colloidal silver gel.

Materials and methods: The film was prepared from PVA, CMC, glycerol and colloidal silver gel by solution casting. Cytocompatibility was assessed using 3T3 fibroblasts with an MTT assay at 48 and 72 hours and fluorescence-based live/dead staining. Mass loss was assessed by incubating pre-weighed specimens in phosphate-buffered saline (PBS; pH 7.4) containing lysozyme at 37°C for up to 72 hours. The source material supplied for this revision contained graphical results but did not provide replicate-level raw data or complete experimental parameters.

Results: Values estimated from the supplied MTT graph indicated approximately 80% cell viability at 48 hours and approximately 95–100% at 72 hours for the experimental group. Values transcribed from the supplied mass-loss graph were approximately 0%, 8%, 15%, 27%, 41–42% and 57% at 0, 6, 12, 24, 48 and 72 hours, respectively. These values are approximate graphical estimates and should not be treated as validated quantitative results until checked against the original data. The fluorescence panels in the supplied figure were not interpretable in the provided document.

Conclusion: The supplied graphical results suggest that the film maintained fibroblast metabolic activity over the reported observation period and underwent progressive mass loss under the tested in-vitro conditions. Incomplete methodological details, unavailable replicate-level data and non-interpretable fluorescence images limit the strength of these findings. Verification using original experimental records is required before journal submission.