Exploration of Microbial Culture Techniques For Microbial-Induced Calcite Precipitation in Civil Engineering Applications

Abstract

Microbial-induced calcite precipitation (MICP) holds promise for revolutionizing civil engineering practices, particularly in soil stabilization and ground improvement. This paper presents a detailed exploration of microbial culture techniques crucial for understanding and replicating MICP experiments, catering to civil engineers unfamiliar with biological methodologies. Bacillus subtilis, chosen for its non-hazardous nature, serves as the primary organism, cultured under controlled conditions. The preparation of Luria Agar (LA) and Luria Broth (LB) media is meticulously outlined, along with bacterial colony cultivation through streaking and spreading techniques. Spectrophotometry facilitates precise cell density quantification, while urease tests differentiate urease-positive organisms. Microscopic examination provides visual insights into bacterial morphology. Centrifugation enables efficient cell harvesting, while stock preparation ensures long-term preservation. Common challenges in bacterial culture are addressed, emphasizing the importance of quality control measures. Through interdisciplinary collaboration, this study bridges microbiology with civil engineering, fostering innovation and sustainable practices.