Gas Chromatography - Mass Spectrometry (GC-MS) Profiling and Phytochemical Characterization of Bilva Taila A Traditional Formulation For Badhirya

Abstract

Background: Badhirya is one among the 28 Karnagata rogas. As per WHO, by 2050 one in every ten people will be affected by hearing loss. This itself shows the importance of treating hearing loss. As per Ayurveda Badhirya is of two types Vataja and VataKaphaja. The basic line of management includes Snehana and Rasayana. Karnapurana (instillation of medicine into external auditory canal) is a major treatment style adopted by the practitioners for treating Badhirya if tympanic membrane is intact. Bilvataila is widely used in practise and it is directly indicated for both types and successful results are available. Aim:To prepare Bilva taila, ensure its safety and identify the phytoconstituents present in it. Objective: To prepare Bilva taila according to Taila Kalpana and to analyse its Physico-chemical parameters, test for heavy metals and microbes for ensuring safety and purity of drug and also to conduct the Gas Chromatography Mass Spectrometry analysis of Bilvataila and thereafter analysing the compounds for their role in treating hearing loss. Materials and methods: GCMS analysis is carried out at Amrith Labs with instrument Shimadzu and identified compounds pharmacological action was collected from various other articles and textual references. Result and Discussion: The physiochemical analysis of the drug was comparable with API standards. Presence of heavy metals was as per permissible limits, no detectable microorganisms in microbiological testing GCMS analysis identified 50 compounds. Oleic acid butyl ester constituted an area of 56.91% followed by Octadecanoic acid ,3-hydroxy-2-tetradecyl methyl ester 17.68%. The identified compounds mainly act as anti-microbial, anti-inflammatory, antidegenerative, anti-oxidant and neuroprotective. Conclusion.All the analysis reports suggest that the drug is having API standard and is safe for use.